TCB Publications - Abstract
Jen Hsin, Chris Chipot, and Klaus Schulten. A glycophorin A-like framework for the dimerization of photosynthetic core complexes. Journal of the American Chemical Society, 131:17096-17098, 2009. (PMC: 2792896)
-helix protein, PufX, and forms a (RC-LH1-PufX)
dimer. To this
date, no high-resolution structure has been reported for the
entire core complex. In particular, the location of PufX within the (RC-LH1-PufX)
dimer is still the subject of much debate. Here, one of
the proposed locations for PufX, requiring its dimerization, is examined. The PufX-dimer
model on the basis of the glycophorin A (GpA)
dimer was constructed, and its robustness was probed through a series of molecular
dynamics (MD) simulations. The free-energy change due
to the replacement of Gly35 by valine was also determined to assess whether this
mutation is responsible for distinct PufX oligomerization
states in different Rba. species. The present study shows that PufX helices form a
stable GpA-like dimer with a helix-helix crossing angle
that could constitute the molecular basis of the reported highly bent and V-shaped
structure of the Rba. sphaeroides core complex dimer.
Download Full Text
The manuscripts available on our site are provided for your personal use only and may not be retransmitted or redistributed without written permissions from the paper's publisher and author. You may not upload any of this site's material to any public server, on-line service, network, or bulletin board without prior written permission from the publisher and author. You may not make copies for any commercial purpose. Reproduction or storage of materials retrieved from this web site is subject to the U.S. Copyright Act of 1976, Title 17 U.S.C.
Download full text: Journal, Request a Copy
